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Selasa, 11 September 2012

Immuno-elektron mikroskopis kuantifikasi klorofil thefucoxanthin, a / c polipeptida mengikat Fcp2 Fcp4, dan Fcp6 dari Cyclotella cryptica tumbuh di bawah intensitas cahaya rendah dan tinggi

RINGKASAN

Diatom Cyclotella cryptica yang tumbuh di bawah cahaya rendah dan tinggi intensitas putih 50 dan 500 foton umol m-2 s-1, masing-masing. Western imunoblotting menunjukkan bahwa diatom diadaptasi cahaya-panen aparatnya, sehingga menimbulkan jumlah yang berbeda dari klorofil yang berbeda fucoxanthin a / c polipeptida mengikat (FCP). Jumlah Fcp2 adalah sekitar dua kali lipat lebih tinggi di bawah cahaya rendah daripada di bawah kondisi cahaya tinggi, sedangkan jumlah Fcp6 meningkat empat sampai lima kali lipat di bawah kondisi cahaya tinggi. Untuk Fcp4, tidak ada perbedaan yang signifikan yang terdeteksi sebagai respon terhadap kedua rezim cahaya. Sel dari Cyclotella tumbuh di bawah intensitas tinggi dan cahaya rendah menjadi sasaran mikroskop immunoelectron. Kuantifikasi label emas, dinyatakan sebagai partikel emas per μm2, mengkonfirmasi hasil yang diperoleh Western imunoblotting. Paparan cahaya rendah mengakibatkan deteksi kira-kira enam kali lebih partikel emas Fcp2-terikat per μm2 dalam membran tilakoid, sedangkan pada sel tumbuh di bawah cahaya tinggi jumlah Fcp6-terikat partikel emas meningkat sepuluh kali lipat. Untuk Fcp4, jumlah yang sama partikel emas per μm2 dihitung di bawah dua rezim cahaya. Hasil ini dikonfirmasi immunocytochemical data molekuler yang berasal dari analisis filogenetik dari urutan gen penyandi klorofil fucoxanthin a / c polipeptida mengikat (fcp gen) dan dari pengukuran konsentrasi steady-state mRNA fcp. Hasil menunjukkan bahwa Fcp2 dan Fcp6 menumpuk di bawah intensitas rendah dan cahaya tinggi, masing-masing, sedangkan Fcp4 tampaknya konstitutif disintesis. [Int Microbiol 2006; 9 (1) :29-36].

Key words: Cyclotella · Bacillariophyceae · photosynthesis · light-harvesting complexes · immunogold-labeling electron microscopy

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Selasa, 03 Juni 2008

PENGKAJIAN KAPANG ENDOFIT DARI TAMAN NASIONAL GUNUNG HALIMUN SEBAGAI PENGHASIL GLUKOAMILASE

Ruth Melliawati*, Ricky Setiadi Suherman**, dan Bambang Subardjo**
* Pusat Penelitian Bioteknolgi LIPI, Jl. Raya Bogor KM.46 Cibinong, Bogor
** Univ. Jenderal Soedirman Jl. Dr. Soeparno, Karangwangkal, Purwokerto

ABSTRACT

Production of glucoamylase generally used through the fermentation-using microorganism. One of microorganism source which never been studied are from endophyte fungus. The purpose of this research is to study the potential microbes of Gunung Halimun Nasional Park (GHNP) for glucoamylase production. Thirty-seven isolate of endophyte fungi has been investigated for the ability of glucoamylase production on PSA (Potato starch agar) media with the strength of clear zone and colony. The potential isolates inoculated to Czapek media to produce glucoamylase on 50 ml scale and measured its activity every 24 hours of incubation time for 96 hours. The best isolate then was reproduced on the larger media (100 ml), and resumed with filtration and ultra-filtration. The enzyme activity, specific activity, and degree of protein was measured in every phase. Selection of amylolitic strength resulted that HL.110F.488 produced the highest amylolitic strength with halo size of 10.47 cm2 , or equal with hydrolysis of starch of 0.0494 gram for 96 hours, while both isolate HL.44F.199 and HL.45F.205 had low amylolitic capacities but a very wide of colony growth, each 38.54 cm2 and 30.76 cm2. Isolat HL.44F.199 produced the highest enzyme activity of 5452.633 unit at 72 hours fermentation, while isolate HL.45F.205 with 4725.58 units at 72 hours fermentation and isolate HL.110F.488 with 3167.609 units at 96 hours fermentation. Glucoamylase has been reproduced by isolate HL.44F.199 on volume media 100 ml. The results show that enzyme activity is 4197.10 units with specific activity 2851.44 U/mg proteins, both get an increasing result after filtration and ultra-filtration reach out 5910.86 units and 4534.45 U/mg proteins.

Keywords: endophyte fungus, glucoamylase production

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Aspergillus species identification in the clinical setting

S.A. Balajee1*, J. Houbraken2, P.E. Verweij3, S-B. Hong4, T. Yaghuchi5, J. Varga2,6 and R.A. Samson2

1Mycotic Diseases Branch, Centers for Disease Control and Prevention, Atlanta, Georgia, U.S.A., 2CBS Fungal Biodiversity Centre, Utrecht, The Netherlands; 3Department of
Medical Microbiology, Radboud University Nijmegen Medical Center and Nijmegen University Center for Infectious Diseases, Nijmegen, The Netherlands; 4Korean Agricultural
Culture Collection, Suwon, Korea; 5Medical Mycology Research Center, Chiba University, Chiba, Japan; 6Department of Microbiology, Faculty of Sciences, University of Szeged,
H-6701 Szeged, P.O. Box 533, Hungary

Correspondence: S. Arunmozhi Balajee, fir3@cdc.gov

Abstract: Multiple recent studies have demonstrated the limited utility of morphological methods used singly for species identification of clinically relevant aspergilli. It is
being increasingly recognised that comparative sequence based methods used in conjunction with traditional phenotype based methods can offer better resolution of species
within this genus. Recognising the growing role of molecular methods in species recognition, the recently convened international working group meeting entitled “Aspergillus
Systematics in the Genomic Era” has proposed several recommendations that will be useful in such endeavors. Specific recommendations of this working group include the use
of the ITS regions for inter section level identification and the β-tubulin locus for identification of individual species within the various Aspergillus sections.


Key words: Emericella, molecular phylogeny, pathogenic aspergilli, polyphasic taxonomy, section Aspergillus section Terrei, section Usti.

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