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Abstrak: Citrullus colocynthis (Linn.) Schrad. merupakan tanaman obat yang penting milik keluarga Cucurbitaceae. Flavonoid "quercetin" diisolasi dari in vivo (daun, batang, buah dan akar) dan kalus in vitro spesies. Sampel dikeringkan secara terpisah Soxhlet diekstraksi dalam metanol 80% dan kemudian reextracted dengan petroleum eter, dietil eter dan etil asetat. Fraksi dipekatkan dan sasaran TLC. Nilai Rf dari quercetin terisolasi dan standar kuersetin dihitung. Bahan dimurnikan menjadi sasaran spektrum IR, HPLC dan diidentifikasi sebagai "quercetin". Penelitian ini juga penting praktis karena quercetin merupakan unsur penting dari Citrullus colocynthis.
Kata kunci: Citrullus colocynthis, Flavonoid, In vivo dan in vitro, Quercetin.
Isolation and Identification of Flavonoid "Quercetin" from Citrullus colocynthis (Linn.) Schrad.
Abstract : Citrullus colocynthis (Linn.) Schrad. is an important medicinal plant belonging to family Cucurbitaceae. Flavonoid "quercetin" was isolated from in vivo (leaf, stem, fruit and root) and in vitro callus of the species. The dried samples were separately soxhlet extracted in 80% methanol and then reextracted with petroleum ether, diethyl ether and ethyl acetate. The fraction was concentrated and subjected to TLC. The Rf value of isolated quercetin and standard quercetin was calculated. The purified material was subjected to its IR spectra, HPLC and identified as "quercetin". This study is also of practical importance because quercetin is an important ingredient of Citrullus colocynthis.
Key words : Citrullus colocynthis, Flavonoid, In vivo and in vitro, Quercetin.
Sumber : http://ajes.in/PDFs/08-1/17.%20Isolation%20and%20identification.pdf
1 Response to "Isolasi dan Identifikasi Flavonoid "Quercetin" dari Citrullus colocynthis (Linn.) Schrad."
saya mahasiswa dari Universitas Islam Indonesia
Nice post, thanks for share :)
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